Stable hollow polyelectrolyte capsules were produced by means of the layer-by-layer assembling of poly(allylamine), PAH, and poly-(styrenesulfonate), PSS, on melamine formaldehyde microcores followed by the core decomposition at low pH. These capsules are nonpermeable for urease in water and become permeable in a water/ethanol mixture. The capsules were loaded with urease in water/ethanol mixture and then resuspended in water. The urease molecules are kept in the capsule, whereas the small urea molecules rapidly diffuse through the capsule wall providing a substrate for the biocatalytic reaction.

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