The object of this study was determination of organochlorine pesticide residues in freshwater samples collected along the Nile River at the Rosetta branch at Shubra Khait region El-Behera Governorate and Kufr EL-Zayat region, EL-Gharbia Governorate from two sites in different seasons during 2016-2017. The determination of organochlorine pesticide residues (PP-DDT, endosulfan, endrin, PP-DDD, OP-DDE, OP-DDD, Aldrin, Heptachlor-epoxide, heptachlor, d-HCH, a-HCH, g-HCH, dieldrin, PP-DDE, and methoxychlor) carried out by GC-µECD. The results indicated that the concentrations of pesticide residues monitored was below the permissible limits according to the World Health Organization (2011). Therefore, it is recommended to preserve the water of the Nile River by not dumpling sewage, agricultural and industrial waste without treatment in the Nile water and using pesticides in good way.