Stimulating additives with protein fillers in the beekeeping where use frames can become an effective biological and technological lever not only in increasing the productivity of bee colonies, but also in the cultivation of complete off spring in subsequent generations of bees. The purpose of the research was to study the amino acid content of working individuals in bee families against the background of stimulating additives in combination with cobalt sulfate and a mixture of “Nanny 2 with prebiotics”. The experiment has been carried out in the educational and experimental apiary of the Russian State Agrarian University – Moscow Agricultural Academy named after K. A. Timiryazev and the Association of beekeepers in the Samarkand region. The object of the research was the bee families of Carpathian breed, which were kept in 16-frame beehives, beds on frames measuring 435×300 mm. It has been found as the result of the research that in order to ensure the secretory function of the pharyngeal glands in the hemolymph of 9-day-old worker bees, which are nurse bees, the level of lysine should be 2,37–2,79 times higher compared to overwintered workers bees, histidine – 2,47–2,85 times, glycine – 2,32–2,46 times, aspartic amino acid – 1,81–1,94 times. The most intensive increase in the level of essential and interchangeable amino acids occured in the hemolymph of working individuals of the spring generation, compared with overwintered bees of the autumn generation. This happened against the background of stimulating additives with honey soap, containing the milk mixture “Nanny 2 with prebiotic” or with cobalt sulfate.