Статья посвящена разработке дифференциально-диагностической среды для идентификации бактерий вида Bordetella petrii. Разработанная селективная среда позволяет выявить бактерии B. petrii в течении 48-72 часов. Разработанные питательные среды специфичны по отношению к бактериям-ассоциантам других видов и родов. Оптимальным составом среды является (на литр): глюконат натрия -20 г, гидролизат казеина-3 г, дрожжевой экстракт-1 г, калий фосфорнокислый однозамещенный -1г, бромтимоловый синий-0,08 г, агар бактериологический-15 г, цефалексин-0,02 г. Показатель чувствительности среды составляет около 100 микробных клеток.

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The article is concerned with the study of main biological properties of bacteria B. trematum species. In this work the research results of their tinctorial, cultural and biochemical properties are shown, with the view to grounding for setup scheme of isolation and bacteriological test of stated microorganism. According to obtained data bacteria of the given species represents gram-negative, coccoid bacillus that are able to grow both on usual and differentially diagnostic mediums. Bacteria B. trematum grow in temperature range of 17-420С, optimal temperature of cultivation is 37˚С. It should be noticed that the most relevant medium for cultivation is bordetellagar It was established that bacteria grow on the sodium chloride mediums in the range of 3-5%. It was found that studied culture of bacteria B. trematum shows проявляет asugarlytic properties, has positive reaction on catalase, negative reaction on cytochrome oxidase and DNAse. Also during the research it was established that bacteria B. trematum doesn’t utilize urea and citrate, has weak proteolytic activity and doesn’t utilize a number of amino acids. Similar results were obtained when using the set Api 20 E and NEFERMtest 24. Additionally it was established that B. trematum doesn’t reconstruct nitrates to nitrites, doesn’t enzyme β-galactosidase, adrinin hydrolase, triptophane dyaminaze, lysine decarboxylase, ornithine decarboxylase and urea doesn’t utilize citrates, doesn’t produce H2S, indole and acetone, doesn’t oxidate sucrose, melibios, amigdalin . Research results represented in the article provide the basis of isolation scheme and identification of bacteria of given species.

The article is devoted to the study of proteomes of poorly studied representatives of genus Bordetella. According to the results of the study, molecular masses of and protein masses of bacteria of genus Bordetella were extracted. In the NCBI system, in accordance with the data obtained by comparing genomes of Bordetella genus, an in-silico analysis of the correspondence of annotated proteomes was performed and the idea was identified by proteome of these species.

Currently, the development of methods for isolation, indication and identification of Pseudomonas stutzeri bacteria from environmental objects and pathological material is an urgent problem. At the same time, there are no data in the scientific literature on the sensitivity of Ps.stutzeri bacteria to various inhibitors, which are necessary for the development of a selective and differential nutrient medium for them. The article presents the results of studying the sensitivity of Ps. stutzeri and their associates to inhibitors such as sodium benzoate, SDS, nalidixic acid, potassium tellurite and sodium azide, which will be used to develop a selective and differential culture medium for Ps.stutzeri bacteria.