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The paper presents the results of an experimental study on the effect of a variable frequency-modulated electric field on the course of a wound process in a burn wound. The evaluation of the effectiveness of the antimicrobial action of wound healing agents against the background of an alternating frequency-modulated electric field has been carried out. The bacteriostatic effect of some wound healing agents used in combination with electrical physical and antiseptic action has been proven.

Background Scientists around the world are interested in applying physical phenomena to various fields of science. The development of entire areas united by one concept (‘plasma medicine’, etc.) stands as a bright example. Present work focuses on only two concepts of interest, which are plasma jet and electric field, as far as results of their combined use in clinical practice and in surgery remain understudied. Objective Experimental evaluation of the effectiveness of physical methods of treatment in skin wound healing. Material and methods The study was carried out on 45 small laboratory animals (rats). They were divided into 5 groups of 9 individuals in each according to method chosen for experimental wound healing. To generate the plasma jet, a portable generator was used, which is the original development of specialists of the Peter the Great St. Petersburg Polytechnic University. Planimetric and histological measurements were analysed. The antibacterial effectiveness of methods under research was investigated using Staphylococcus aureus 209P, cultivated in vitro on 6- and 12-millimeter discs. Discussion The combined use of non-thermal atmospheric pressure plasma and frequency-modulated electric field signal exhibited the ability to accelerate eschar separation by 52.1% (p < 0.05) and stimulate restorative regeneration by 56% (p < 0.05). Combination of physical methods of treatment demonstrated pronounced antibacterial effect. A histological examination on biopsy sample on the 21st day of observation revealed formed (mature) granulations and large number of newly formed vessels. The cellular composition of epidermal basement membrane showed high level of differentiation. Conclusion The data obtained suggest that the use of the proposed methods in clinical practice will improve the treatment process of the patients with wounds of different etiology.

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