The experiments were conducted in the Laboratory of Tissue culture/Department of Forestry/College of Agriculture and Forestry/the University of Mosul. To propagate three citrus rootstocks (Citrus aurantium L., Citrumelo swingle, and Cariso 35 (C35))In vitro. During the Initiation stage, the single nodes were planted on a solid MS medium supplemented with five concentrations of BA (0, 1, 2, 3, and 4 mg L-1). After four weeks, the best significant percentage of response for Cariso35 and Citrumelo swingle, while the highest value of BA effect was at 2 mg BA L-1(96.67%). The No. of shoots there are nonsignificant differences, While the best No. of the shoot (1.41) obtained at 2 mg BA L-1, The highest significant shoot length (2.14cm) was obtained at Cariso35 compared with other rootstocks, so that, 1mg BA L-1 was achieved (2.01 cm). At the multiplication stage, Citrumelo swingle achieved the highest significant value (2.68shoots), whereas adding 4 mg BA L-1 gave the best significant No. shoots (3.10). as soon as, the shoot length (1.03cm) was obtained for Cariso35, while control treatment achieved a significant increase in shoot length (1.30cm) which differ significantly from other treatments. The highest significant number of leaves was (11.50 leaves) for Citrumelo swingle, while BA at 2 mgL-1 was dominated value achieved at 11.00 leaves. In the rooting stages, the best rooting percentage was 60% for Cariso 35 cultured on MT solid medium, but a non-significant difference between all IBA concentrations. While the significant No. of roots was 3.28 obtained from Citrumelo swingle, and the concentration 1.5 and 2 mg IBA L-1 achieved the highest significant values (2.90 and 2.24 root) respectively. Citrumelo swingle and the 1.5 mg IBA L-1 gave the shortest significant duration to formed roots which were 21.56 and 18.60 days respectively.
To obtain further information about the effect of some growth regulating substances upon bunch weight and fruit quality of Samani date cultivar (12-year-old) were assessed for two successive seasons (1997 & 1998), effects of different concentrations of IAA + BA and GA3 + BA applied twice one week after pollination and two weeks later were compared on different bunches on the same palm.Both bunch and fruit fresh weight were increased by IAA + BA application especially 10 ppm IAA + 10 ppm BA treatment, followed by GA3 + BA treatments especially 5 ppm GA3 + 10 ppm BA. Whereas the percentage of seed weight was decreased with (5 ppm IAA + 10 ppm BA) treatment.The IAA + BA treatments increased fruit length and diameter especially with 10 ppm IAA + 10 ppm BA treatment, followed by GA3 + BA treatments especially 5 ppm GA3 + 10 ppm BA. Concerning L/D ratio, no significant differences were obtained between treatments with the exception in the first season where the treatment 10 ppm IAA + 10 ppm BA decreased it.TSS, reducing and total sugars were increased by IAA + BA treatments especially (10 ppm IAA + 10 ppm BA) treatments and GA3 + BA treatments especially 5 ppm GA3 + 10 ppm BA and were decreased by elevating the rate of GA3 + BA application.The results of this experiment suggest the benefits of the application of IAA + BA to improvebunch weight and quality of Samani date fruit.
To obtain some information about the effect of some growth regulating substances on produced normal size seedless fruits, yield per bunch and fruit characteristics at full yellow colour stage of Samani date cultivar (12-year-old) were assessed for two successive seasons (1997 and 1998). Effects of different concentrations of IAA, GA3 and BA applied twice during the full bloom and one week later were compared on different bunches on the same palm.Unpollinated bunches treated with IAA and/or GA3 twice at 5,10 and 20 plus 10 ppm BA produced seedless fruits. Yet, the yield was significantly less than that of seeded fruits developed after hand pollination. Seedless IAA, GA3 and BA induced fruits were lower in average fruit weight, fruit size, seed weight, fruit length, fruit diameter and length/diameter ratio. Whereas, higher values of total soluble sugars and reducing sugars were induced compared to those produced after hand-pollination. The better effects were obtained with GA3 + BA application (20+10 ppm) and IAA + BA application (20+10 ppm).Results concluded that production of seedless fruits is possible and further studies should be done to produce fruits with normal size.
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