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Acute ischemia was induced in the lower limb of growing dogs by injecting the femoral artery with a suspension of carborundum and ground glass in physiological saline. This resulted in the loss of the cellular pattern and the cartilaginous matrix in the growth cartilage. The production of myofibrillike material was observed only in the growth cartilage as well as in the intertrabecular spaces of metaphysis in the injected leg.

Afree flap is a composite block of tissue transferred from a donor site to a distant recipient site, where its circulation is restored by microvascular anastomosis of the artery and vein in the vascular pedicle to vessels at the recipient site. Indications for free flap transfer are trauma with soft tissue loss, e.g. lower third tibulo-fibular compound fractures, and reconstruction of complex defects following oncological surgery and burns. Free flaps allow better matching of resected tissue and provide more even cover of the defect with superior aesthetic and functional results, making them the preferred option for reconstruction. Adequate microvascular perfusion is vital for flap survival and decreased blood flow with consequent ischaemia is one of the main causes of flap failure.

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