Enzyme‐linked fluorescence assay (ELFA) was evaluated for detection of lettuce mosaic virus (LMV). The assay, which could detect 1 ng/ml of purified virus, was approximately equivalent in sensitivity to results obtained with biotin‐avidin ELISA. The immunoassay detected LMV antigen in contrived seed lots containing LMV‐infected seeds where virus concentrations were presumably at low levels. Zusammenfassung Die Anwendung eines enzymgekoppelten Fluoreszenzassays (ELFA) bei der Entdeckung des Lettuce Mosaik Virus in Salatsamen Ein enzymgekoppelter Fluoreszenzassay (ELFA) wurde auf seine Tauglichkeit bei der Entdek‐kung des Lettuce Mosaik Virus (LMV) geprüft. Der Assay, der 1 ng/ml gereinigtes Virus feststellen konnte, war in seiner Sensitivität mit den Ergebnissen nach Biotin‐Avidin ELISA ungefähr gleich einzustufen. Der Immunoassay entdeckte LMV‐Antigene in Samenproben, die LMV‐infizierte Samen enthielten, bei denen die Viruskonzentrationen aber wahrscheinlich sehr niedrig waren.

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This reproduction was made from a copy of a manuscript sent to us for publication and microfilming. While the most advanced technology has been used to pho tograph and reproduce this manuscript, the quality of the reproduction is heavily dependent upon the quality of the material submitted. Pages in any manuscript may have Indistinct print. In all cases the best available copy has been filmed.The following explanation of techniques is provided to help clarify notations which may appear on this reproduction. Manuscripts may not always be complete. When it is not