A huge number of cell culture strains obtained from one organ (tissue) of an animal, sensitive to causative agents of various infections, are known. However, a comparative characterization of various cell culture strains sensitivity to such bee viruses as SBV has not yet been carried out. This work was carried out in the Department of Cell Biotechnology and the Laboratory of Bee Diseases of the Federal State Budget Scientific Institution “Federal Scientific Centre VIEV”. For the study, we took cell cultures of the African green monkey kidneys (Vero (3 strains) - Vero (strain from the FSC VIEV Collection)), Vero K (strain from the FSC VIEV Collection), Vero (strain from Germany). We have shown that all 3 of the Vero cell cultures above strains are sensitive to SBV, however, the cell model in which the most pronounced signs of CPD were revealed is the Vero K cell line. The first signs of CPD manifestation virus in the culture of Vero K cells were observed at first passage. After 6 days. screeds were found in the monolayer after infection. At the second passage of the virus after 5 days. culturing CPD manifested itself as the appearance of voids, cell strands, and rounding of the cells part.

In the article, based on the results of studies of the effectiveness of the action on the sacbrood virus of bees, it was found that, based on the identification of qualitative signs of infection of the virus under study in the culture of PT cells in the form of the formation of a large number of vacuoles in the cytoplasm, heating to 50-70°C for 10 minutes does not contribute to reducing the virulence of the virus. The death of the virus is observed at a temperature of 100°C for 10 min. The drug BashApiDez (Active substances: hydrogen peroxide 3.5%, alkyldimethylbenzylammonium chloride 1.4%) in a dilution of 1: 100000 is not able to eliminate the infecting ability of SBV. It was also found that the treatment of the sacbrood desease with the drug "Bactopol" does not contribute to its elimination.

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Ðåçþìå. Èñïîëüçîâàíû êëåòî÷íûå êóëüòóðû ðàçíîãî âèäîâîãî è òêàíåâîãî ïðîèñõîaeäåíèÿ äëÿ èçó÷åíèÿ öèòî-ìîðôîëîãè÷åñêèõ èçìåíåíèé, âûçûâàåìûõ âèðóñîì ìåøîò-÷àòîãî ðàñïëîäà. Âûÿâëåíû îäèíàêîâûå öèòîìîðôî-ëîãè-÷åñêèå èçìåíåíèÿ â ðàçëè÷íûõ êëåòî÷íûõ øòàììàõ. Summary. Cell cultures of different species and tissue origin were used to study the cytomorphological changes caused by the sacbrood virus.The same cytomorphological changes were observed in different cell strains.