This study aimed to investigate Micro RNA 34a-5p and Micro RNA 365a-5p in addition to the traditional biochemical analysis as a useful diagnostic biochemical marker for chronic Hepatitis C Virus (HCV). The present study included 17 individuals with chronic liver disease, who admitted to Benha University Hospital. After investigating 5 patients from all patients, were found to be positive polymerase chain reaction (PCR) chronic HCV. 10 control negative serum samples were collected from the control healthy group. Biochemical analysis was done for investigating liver function parameters alanine transaminase (ALT), aspartate transaminase (AST), total bilirubin (TBil), direct bilirubin (DBil), gama glutamyl transferase (GGT), alkaline phosphatase (ALP) and albumin (Alb) and hematological analysis including total leucocytic count (TLC), hemoglobuin concentration (Hb) and platelet count (PLT), Alpha feto protein (AFP) as a tumor marker indicator. RNA was extracted from serum samples for gene expression of microRNA34 a-5p and microRNA 365a-5p. HCV group showed a significant increase in liver function parameters (ALT, AST, TBIL, DBIL, GGT, ALP) while there was a significant reduction in the albumin levels. Moreover, there was a significant decrease in TLC with non-significant decrease in the HB concentration and the platelet count. AFP concentration in HCV group was significantly increased compared to the control group. HCV group revealed a significant increase (8.98 ± 0.34) in miRNA34, with a significant decrease in miRNA365 (0.25 ± 0.03). We concluded that miRNA34a-5p and miRNA365a-5p expressions in addition to different biochemical analysis can be used as a useful diagnostic marker for HCV diagnosis.

The oncoplastic surgery (OPS) technique, which was first attempted by Audretsch et al., expands on the concept of breast-conserving surgery [1]. The pedicled latissimus dorsi (LD) flap serves an important function in breast reconstruction, but its utility is limited by its inability to provide sufficient volume [2]. Autologous expansion of the LD fold with lipomove has been utilized to keep away from situation of an embed. We improve the method by performing lipo-move during bosom recreation [3]. The examination was a planned, relative investigation incorporates 30 female patients with bosom malignancy stages, introduced to Benha University Hospital during the period from March 2018 to May 2020.All patients gave bosom knots, both ways sided, the ages at time of introduction went from 36 to 57 years (normal 46 years).they were partitioned into two gatherings as indicated by sort of offered substitution strategy: Group (A): by utilizing smaller than expected latissimus dorsi muscle fold just and Group (B): by utilizing scaled down latissimus dorsi muscle fold notwithstanding quick intraoperative fat joining. As of late, fat uniting has been demonstrated to be a promising restorative methodology for scar the executives after consumes and physical injury. This method can be utilized in the setting of a LD fold/tissue expander/embed recreation, and could likewise be extended to different autologous bosom reproduction choices [4].