Pythium damping-off of Chilli (Capsicum annuum L.) is very common problem in fields and greenhouses, whereas the organism kills newly emerged seedlings. In present investigation repeated isolations from rotten seeds as well as toppled seedlings of Chilli cv. Pusa Jwala have yielded the cultures of P. aphanidermatum. The damping off mainly being soil borne, the primary infections are needed to be controlled with suitable fungicides. The different fungicides and bioagents were evaluated as seed dressers and soil drenching. The result revealed that there was significant difference in per cent disease incidence at 15, 30 and 45 day after sowing. Seed dressing of metalaxyl reduced the damping off disease significantly as compared to all other treatments and at par with thiram. Among bioagents, Trichoderma harzianum was proved effective in controlling the disease. In per cent mortality it was found that metalaxyl reduced the plant mortality significantly over control followed by thiram. Among the bioagents, Trichoderma harzianum was superior over all other treatments followed by T. viride.