Sonchus arvensis L. is a medicinal plant and is mainly taken from nature which over time will cause it to run out. This plant has rosette and inflorescence leaves commonly harvested simultaneously, but they can also be harvested separately. The experiment goal was to identify the leaf characteristics of S. arvensis with different methods of leaf harvest. The experiment used a factorial completely randomized block design. The three accessions of S. arvensis, namely Lembang, Tawangmangu, and Banjararum as the first factor. Harvest methods: gradual and simultaneous harvest of rosette and inflorescence leaves as the second factor. The experiment was carried out at IPB experimental station in Cikarawang, Bogor, Indonesia (± 250 m asl, 6°30’-6°45’ S, 106°30’-106°45’ E), on clay-loam latosol soil. The results showed that the interaction between two factors influenced canopy diameter and the number of rosette leaves where Tawangmangu had the values of those variables were higher with simultaneous- than those with the gradual- harvest. Other leaf properties, namely the number of inflorescence leaves and leaf size of rosette leaves, were different among S. arvensis accessions. In conclusion, rosette and inflorescence leaves of S. arvensis can be harvested gradually or simultaneously.