In this study gold nanoparticles (AuNPs) was prepared by electrolysis method Au thin film which was deposited on a glass substrate by drop-casting technique structural, optical and topographic characteristic of prepared nanoparticles were examined by UV-VIS absorption, TEM electron microscopy, AFM atomic microscopy, and XRD diffraction. Antibacterial activity of nanoparticles has been investigated against many pathogenic bacteria, including Escherichia coli, Staphylococcus aureus, bacillus and Pseudomonas aeruginosa using a well-spread method, and the results showed that nanoparticles have an inhibitory effect against all types of pathogenic bacteria For diseases. And 30 and 25 mm) for aerosols, E. coli, Bacillus subtilis, Staphylococcus aureus and Staphylococcus aureus, respectively.