This paper focuses on the ecological status and diversity indices: Simson’s Index (C), Simson’s Index of Dominance (D) and Shannon-Weaver Index (H) of Dactylorhiza hatagirea and its associates- Rheum australe and Rumex nepalensis in Lete village of Mustang District within the Annapurna Conservation Area. The study was conducted during the monsoon season (June/July) of 2013 in the Lete VDC of Mustang District. The study site possessed an area of 4.5 ha. Altogether, 100 circular plots, each with 25 m2 area, were laid out purposively within the study area; the sampling intensity being 5.55%. The relative frequency, the relative density, the abundance, the relative coverage and the Important Value Index of the species were found to be 61.11, 53.91, 1,061.54, 72.2 and 187.24 respectively. Similarly, the Simson’s Index (C), the Simson’s Index of Dominance (D) and the Shannon-Weaver Index of the species were found to be 0.41, 0.59 and 3.27 respectively, indicating relatively even and relatively diverse community. The study showed relatively higher values of all the parameters of D. hatagirea as compared to its associates indicating good ecological value. However, threats remain due to the illegal harvesting of this valuable orchid and overgrazing in the study site.Banko JanakariA Journal of Forestry Information for NepalVol. 26, No. 1, Page: 45-52, 2016