The confidence with which
fluorescence decay data can be interpreted is dependent upon the accuracy and
precision of these data. This dependence is especially critical when more than
one exponential is involved in the decay.1,2 Unfortunately decay
curve parameters have often been presented without any indication of either
their accuracy or precision. An analysis is presented which suggests that the
covariance ellipsoid is the most satisfactory statistic with which to define
the precision.
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