Purpose. Study of perinatal and neonatal outcomes in newborns born to mothers with preeclampsia.Characteristics of children and research methods. Perinatal outcomes were analyzed in 559 mother–newborn pairs divided into 2 groups: Group 1 included 200 children to mothers with preeclampsia (143 with moderate and 57 with severe preeclampsia). Group 2 was composed of 359 newborns to mothers with a physiological course of pregnancy. The medical history, features of the course of pregnancy, delivery, and the early neonatal period were analyzed. The condition of newborns was assessed on the Apgar scale at 1 and 5 minutes, the assessment of physical development — using centile tables for full-term and premature newborns (INTERGROWTH-21st), the assessment of neuromuscular and physical maturity on the Ballard scale.Results. The study showed that children born to mothers with preeclampsia are significantly more likely to have metabolic and hematological disorders, a higher risk of developing infectious and inflammatory diseases, and a direct correlation with the period of manifestation and severity of preeclampsia. The higher occurrence of these conditions and the structure of pathological changes in the neonatal period led to a longer stay of children of this group after birth at newborn intensive care units, and also required observation at the second stage of nursing.Conclusion. Preeclampsia is a risk factor for neonatal complications. The identified features of adaptation in newborns from patients with preeclampsia and the structure of pathological conditions in the early neonatal period should determine the management tactics and timeliness of treatment and diagnostic measures aimed at improving the provision of medical care to the pregnant woman and subsequently the newborn.

Both the ratio of different leukocyte subgroup content and the leukocyte morphology in peripheral blood of newborns are important in diagnosis of several diseases including combined immunodeficiency and neonatal septicemia. There is a need for development of screening methods for parallel study of the leukocyte morphology and population structure in the newborn peripheral blood. We aimed to determine the relative abundance of different leukocyte subsets and to study their morphology in full-term and premature newborn babies and healthy adult volunteers using the cell-binding microarray – a transparent support with immobilized antibodies against leukocyte cluster-of-differentiation antigens. The work was supported by the Scientific council and approved by the ethical committee of the Centre. We have studied the peripheral blood of 12 full-term newborns (38–40 weeks gestation), 9 premature newborns (22–32 weeks gestation) and 18 healthy adults. The relative abundance of the leukocyte and their morphology were determined using the cell-binding microarray including antibodies against CD2, СD3, СD4, CD5, СD7, CD8, CD10, СD11b, CD11c, CD13, CD14, CD15, CD16, CD19, CD20, CD22, CD25, CD33, CD38, CD41a, CD45, CD45RA, CD45RO, CD61, CD64, CD117, CD123, HLA-DR. The percentage of leukocytes positive for every of the studied surface CD antigens among the peripheral blood mononuclear cells of full-term and preterm newborn babies and healthy adults determined on the cell–binding microarray are in good agreement with published flow cytometry data. CD11b+ leukocytes both in premature and full-term newborns included up to 21% myelocytes and 27% metamyelocytes. The reported data can be used as reference values in cell-binding microarray application in diagnosis of combined immunodeficiency or neonatal septicemia.

Федеральное государственное бюджетное учреждение «Национальный медицинский исследовательский центр акушерства, гинекологии и перинатологии имени академика В.И. Кулакова» Министерства здравоохранения Российской Федерации, 117997, г. Москва, Российская Федерация 2 Федеральное государственное автономное образовательное учреждение высшего образования Первый Московский государственный медицинский университет имени И.М. Сеченова Министерства здравоохранения Российской Федерации (Сеченовский Университет), 119991, г. Москва, Российская Федерация 3 Федеральное государственное бюджетное образовательное учреждение дополнительного профессионального образования «Российская медицинская академия непрерывного профессионального образования» Министерства здравоохранения Российской Федерации, 125993, г. Москва, Российская Федерация