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Bufo bufo verrucosissima has a karyotype consisting of 22 chromosomes (6 pairs of large and 5 pairs of small chromosomes which are meta-and submetacentric). By means of Ag-AS-staining nucleolar organizers were localized in the telomeric region of the long arms of the6th pair of chromosomes. The karyotype differs from those of the other B.bufo subspecies by the form of the 4th pair, which is metacentric. A slight chromosomal polymorphism was shown also after C-banding of B. b. verrucosissima and B. b. bufo chromosomes.

The amounts of nuclear DNA in blood erythrocytes of 18 species of Anura from the USSR have been determined to be in the range of 4.0--20.6 pg (10(-12) g). Brown frogs of genus Rana have lower mean genome sizes than green frogs. Palaearctic Anura, as a whole, have a greater content of nuclear DNA than the species of the same families from regions further south.

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