RESUMO:As técnicas de propagação podem subsidiar o manejo fornecendo alternativa para produtores de plantas medicinais e evitando-se a coleta indiscriminada. Objetivou-se avaliar o tamanho de estacas com e sem folhas na produção de mudas de insulina (Cissus verticillata (L.) Nicholson & C.E. Jarvis). As estacas foram confeccionadas a partir de ramos apicais herbáceos de insulina, com corte em bisel na base e corte reto no ápice; com comprimento de 5, 10 ou 15 cm; mantidas com duas folhas inteiras, duas folhas cortadas ao meio, ou ausência de folhas; sendo desinfestadas com solução de hipoclorito de sódio a 0,5%, por 15 minutos. As estacas foram plantadas em recipientes plásticos com capacidade de 180 mL, utilizando vermiculita fina como substrato e acondicionadas em casa de vegetação sob temperatura de 22 ± 2ºC. O delineamento experimental foi inteiramente ao acaso e as médias foram comparadas pelo teste Tukey ao nível de 5% de probabilidade. Após 60 dias constatou-se que as estacas de 5 cm com folhas inteiras, e aquelas com metade das folhas, e também as estacas de 10 cm com folhas inteiras apresentaram taxas acima de 70% de enraizamento, com as melhores médias para número e comprimento de raízes formadas. Houve elevada taxa de mortalidade para estacas confeccionadas sem folhas. A formação de calo e o percentual de estacas vivas não foram significativos para os tratamentos. Portanto, estacas de 5 cm de comprimento com folhas inteiras ou meia folha, e estacas de 10 cm com folhas inteiras, são as mais adequadas para a propagação vegetativa de insulina. Palavras-chave:Vitaceae, enraizamento, tamanho estaca, presença folhas. ABSTRACT: Vegetative propagation of insulin (Cissus verticillata (L.) Nicolson & C.E.Jarvis) by cutting. Propagation techniques may support handling, this way providing an alternative for producers of medicinal plants, thus avoiding indiscriminate collection. We aimed to evaluate the size of cuttings with and without leaves on the seedling production of insulin (Cissus verticillata (L.) Nicolson & C.E. Jarvis). The herbaceous apical cuttings were prepared with diagonal cut at the bottom and straight cut at the apex, with 5, 10 or 15 cm in length, kept with two whole leaves, two leaves cut in half or without leaves, being sterilized with sodium hypochlorite at 0.5% for 15 minutes. The cuttings were planted in plastic containers with 180 mL capacity with vermiculite as substrate and placed in greenhouse at the temperature of 22 ± 2° C. The experimental design was completely randomized and means were compared by Tukey's test at 5% probability. After 60 days, we found that the cutting of 5 cm with whole leaves and with leaves cut in half and the cutting of 10 cm with whole leaves had rates above 70% of rooting with the best means for number and length of roots. There was a high mortality rate for cuttings made without leaves. The callus and the percentage of live cuttings were not significant for the treatments. Therefore, 5 cm long cuttings with whole or half leaves and 10 cm long cuttings with wh...
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