A B S T R A C TThe aim of this study was ultrasonographic evaluation, subjective and objective or quantitative, of the SDFT core lesion in donkeys treated with intra lesional injection of PRP. Subjective evaluation was performed by assessment of fiber echogenicity score (FES) and fiber alignment score (FAS). Quantitative evaluation was performed by gray scale histogram analysis and measuring of core lesion cross section area (CSA). The present study was carried out on two groups (Group-I) 12 donkeys treated by intra-tendenious injection of freshly prepared (PRP) and (group-II) 7 donkeys as control group and subjected to intra-tendenious injection of normal saline (Placebo). Ultrasonographic assessment were performed before induction, 5 th , 15 th , 30 th , 45 th , 60 th and 90 th days post induction of core lesion. The PRP treated group revealed significant (p ≤ 0.05) improvement of FES and FAS at 15 th , 30 th , 60 th and 90 th days and at 30 th days post induction, respectively. Moreover, there was significant improvement of the core lesion CSA and theme an gray scale histogram during all time points to reach near to the normal value at the end of the study. The mean gray value of the PRP treated group was significant (p ≤ 0.05) higher than that of control group at all-time points of the experiment. The obtained subjective and quantitative ultrasonographic assessments in the present investigations proved that these parameters could be valuable in the evaluation of SDFT core lesions and monitoring the treatment of tendopathy in equines.

A new trend of regenerative medicine was introduced recently in the field of second intension wound healing. The PRP and PRF represented a corner stone in this clinical practice, were it admit many factors for acceleration of wound healing, enhancement of wound epithelization and neovascularization. PRP and PRF are considered as a great concentrated source of growth factors (PDGF, TGF-β, VEGF, bFGF) and cytokines which are fundamental fro wound healing. Nine adult dogs were used in this study; we induced a 3cm diameter full thickness cutaneous injury at the right chest region. The animals were divided into three groups equally and the wounds were treated twice weekly for three successive weeks. Group A received only normal saline (control group). The members of second group treated by PRP (group B) while, the group C treated by PRF. Clinical evaluation, Molecular studies of IL10 & TGF-β and histopathological examination were used to demonstrate the difference between the three treatment regimes. Results showed non-significant negative low correlation between loss of weight and WHR%, and showed significant high positive correlation between treatment cost either by PRP or PRF with IL10 (0.79*) and WHR%(0.996**). We concluded that the PRP and PRF exhibited higher regeneration capacity and accelerate the quality of wound healing. The PRP was more superior to PRF, but not significantly different. The IL10 was significant increase in expression in PRP, while TGF-b was non-significant increased in PRF group

A total number of 114 clinical cases (63 cattle and 51 buffaloes) were included in the current study. These cases were suffering from different varieties of abdominal wall swellings (46 abscesses; 49 hernias and 19 hematomas) and they were subjected to ultrasonographic examination. From the aforementioned cases the unripend abscess appeared ultrasonographically as a circumscribed echogenic structure with mild distal acoustic enhancement while the ripened abscess appeared as anechoic to hypoechoic structure representing watery to creamy pus contents. The ripened abscess with chronic fibrotic omphalitis revealed a hyperechogenic band with an evident acoustic shadowing. Recent hematomas appeared as anechoic area (blood content) divided by a thin echogenic septum into small chambers toward its center, while old hematoma revealed anechoic to hypoechoic appearance divided by a thick echogenic septum. Reducible hernias revealed disruption of the abdominal wall continuity with intestinal loops and its characteristic peristaltic movement and anechoic, hypoechoic to echogenic contents while irreducible hernias characterized by an evident anechoic inflammatory exudates; reduced peristaltic movement and thick hernial sac with echogenic fibrous adhesions between the sac and the abdominal wall. In conclusion, ultrasonography provides an accurate, non-invasive and fast diagnostic tool for different abdominal wall swellings in cattle and buffalo.

The aim of this study was to assess the effect of platelet rich plasma (PRP) on the healing of created core lesions in the superficial digital flexor tendon (SDFT) in donkeys. Serial histopathological specimens were taken from normal and at 5th, 15th, 30th, 60th and 90th days post PRP treatment. Histopathological findings showed early rapid healing process with early angiogenesis, increased fibroblastic condensation, the nuclei appeared more elongated, flattened arranged in the peripheral collagen fibers. The collagen fibers changed from randomly arranged to semi-parallel and finally highly reoriented parallel closely packed collagen fibers at 90th days of PRP treatment. Compared to saline control displayed few scattered fibroblasts and angiogenesis. As well as less regularly arranged collagen fibers. Histopathological evaluation 90 days after three successive PRP treatment proved a more rapid tendon tissue reconstruction and maturation similar to the healthy tendons.

Background: This study involves the use of a new multifunctional prosthetic mesh for treatment of the perineal hernia without complications. The prosthetic mesh is a hybrid platform of both synthetic and natural materials, mainly consisting of a synthetic commercial polyester fabric (CPF) to deliver the required mechanical integrity. The CPF mesh was coated by a natural biodegradable, biocompatible, and antimicrobial layer of chitosan incorporating phenytoin-loaded pluronic nanomicelles for healing promotion and ciprofloxacin-alginate polyelectrolyte complex-based microparticles as antibacterial agent. Aim: To evaluate the new developed multifunction polyester-based hybridmesh to repair perineal hernia clinical cases in dogs. Methods: The used multifunctional prosthetic mesh is a hybrid of the natural biodegradable, biocompatible, and antimicrobial material used in six cases with perineal hernia. Results: The prosthetic polyester-based hybrid mesh was found very helpful in repairing clinical cases of perineal hernias in six dogs without unwarranted other surgical procedures or complications. The developed mesh proved its feasibility in terms of efficient biocompatibility, stability, sterilizability, and low cost. Conclusion: The prosthetic commercial polyester-based mesh provided the ideal and feasible alternative prosthesis with many advantages.