SUMMARYThe monoclonal antibodies (MAbs) against lipopolysaccharide of virulent strain of Legionella pneumophila serogroup 1 were produced. Three most productive hybrid clones (5F4~ 5F10 and 2C9) were selected from fusions of mouse myeloma cells with spleen cells from BALB/c mice, immunized with bacterial outer membrane antigens. All generated clones were IgGsecreting. The MAbs had narrow strain specificity and showed no cross-reactions with other unrelated bacterial species. These antibodies were tested in sandwich ELISA. The results suggest that the MAbs could be used for diagnostic purposes.

Monoclonal antibodies (MAb) were produced against two B. pseudomallei high-purified membrane proteins with Mr 29 kDa (p29) and 45 kDa (p45). Monoclonal antibodies from culture supernatant fluids of 4F2 and 1G11 clones showed specific interaction with protein moiety of p29 both Burkholderia pseudomallei and Burkholderia mallei in ELISA and Western blotting. However, MAb of 3G4 clone were bound to the LPS-protein structures of these microbial cells. Analysis of interaction of Mabs from 4F2 and 1G11 clones with antigens of different lysates of pathogenic cells confirmed high specificity of these antibodies to p29 membrane protein of B. pseudomallei and B. mallei.

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