The stage of introducing explants into a sterile culture is difficult in the technology of clonal micropropagation of plants. The article shows the possible ways of sterilization and the introduction terms of explants of the clonal apple stocks 54-118 into in vitro culture in order to reduce planting infection and increase the yield of sterile viable explants. The best time for introducing clonal apple stocks 54-118 into sterile in vitro culture is the period of active shoot growth. Sterilization of explants with ethyl alcohol (70,0 %, 1 min) in combination with hydrogen peroxide (33,0 %) for 7 minutes and ethyl alcohol (70,0 %, 1 min) in combination with diacide (0,1 %) within 6 minutes contributed to the production of 63,0 % and 60,0 % of viable sterile explants.

Clonal micropropagation of plants is a modern popular method of vegetative reproduction, which allows to obtain high-quality planting material in a large volume in a short time. The research is devoted to the technology of clonal micropropagation of the rose Reine Sammut, in particular, optimization of the nutrient medium composition. To increase the efficiency of micropropagation, the silicon-containing preparation Siliplant was added to the composition of the nutrient medium according to the Murashige-Skoog (MS) recipe as an alternative to the complex of trace elements. At the stage of the actual micropropagation with the combined use of 6-benzylaminopurine (6-BAP) at doses of 1.0; 2.0 and 3.0 mg/l and Siliplant at doses of 1.0; 2.0 and 3.0 ml/l, no large differences were found. Nevertheless, the inclusion of Siliplant (1.0 ml/l) and 6-BAP (3.0 mg/l) in the medium contributed to an increase in the reproduction factor by 3.1 pcs./cuttings at LSD05 = 1.0 pcs. In addition, Siliplant at a dose of 1.0 ml/l as part of a hormone-free nutrient medium MS contributed to a significant increase in shoot growth (by 4.2 mm at LSD05 = 3.6), and at a dose of 3.0 ml/l stimulated the development of standard micro-plants of roses.

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