It has been found that the cup plant green mass is a promising substrate to culture yeasts, including Saccharomyces cerevisiae. A comparative study of efficiency to use the dried and intact green mass of this plant as a component of the medium for yeast cultivation was carried out. In the Republic of Ossetia-Alania, S. perfoliatum is a perennial, high-yield introduced species, yielding up to 1500 cwt of green mass per hectare. Using the cup plant green mass in to grow yeasts is economically beneficial, due to its high nutrient content. Key words: yeasts, culture media, cup plant, hydrolysis, biomass Funding - The work was financed by the extra-budgetary fund of the Gorsky State Agrarian University.