Both patients and hospitals are harmed by hospital-acquired infection, sometimes known as “nosocomial infection.” To prevent these hospitals, require excellent disinfection and sterilization technologies, which are now integrated into a single department known as the central sterile supply department (CSSD).Aim is to study Organization, workload and the Quality Control Practices in CSSD. This descriptive, observational and hospital survey based study was carried out over a period of 6 months from November to April in Santosh Medical College & Hospital Ghaziabad. Monitoring of CSSD is done to oversee the use of different sterilization methods and capturing indicators to monitor sterilization process and ensure technical maintenance of the equipment according to National standards and manufacture’s recommendation. CSSD of the institution is centrally located within the hospital. Various articles including linen, dressings, surgical instruments and trays are sterilized in the CSSD. Sterilization is done by steam sterilization and gas sterilization with a total of seven autoclaves and two ETO machines. Physical, chemical are regularly used during the process of sterilization. CSSD of institute with proper practices of sterilization methods helps in continuous improvement required to decrease hospital acquired infections (HAIs).