One of the plants that is well known in the community and is used as a traditional medicine is patchouli (Pogostemon cablin Benth.). It contains essential oils, flavonoids, saponins, tannins, glycosides, terpenoids and steroids. The alcohol content, such as patchouli alcohol and its derivatives, phenol, and a group of terpenoids in patchouli oil has antibacterial activity. The aim at this research to determine the total phenolic condition the ethanol extract from the patchouli leaves. The extract obtained was analyzed ist content using UV-Vis spectrophotometer. The measurements were done at the maximum wavelength of 662,85 nm. The result showed that the total phenolic in patchouli leave is 327,84 mgGAE /gram of extract.

This research is about antioxidant activity analysis of Noni products syrup (Morinda citrifolia L.) using DPPH (1,1diphenyl-2-picrylhydrazil) method. The research aimed to analysis of antioxidant activity of two different brands of Noni products syrup using spectrophotometer UV-Vis. This analysis using quarcetin as standard to determine antioxidant activity and DPPH reagen as radical sources. Quarcetin and Noni products syrup are made five series concentrations in metanol. One ml of each concentration added 4 ml DPPH 50 µM and then added metanol up to 10 ml in flask. Then incubated for 30 minutes at room temperature and protected from light. Measurement of absorbance using spectrophotometer UV-Vis at 514 nm. The IC50 values were 25.7 µg/mL for brand A, and 9.839 µg/mL for brand B.

Sunlight contains UV rays that adversely affect the skin. These adverse effects can be minimized by the use of UV protective materials, both natural and synthetic. Soursop fruit (Annona muricata L.) has a high content of polyphenol compounds, some vitamins such as vitamin C, B1 and B2. This study aims to determine the activity of UV light protection on soursop juice (Annona muricata L.) by using Sun Protection Factor (SPF) calculation. The measurement of SPF values using the UV-Vis spectrophotometric method in the wavelength range from 290-320 nm. The results showed that soursop juice (Annona muricata L.) had UV light protection activity at 1% concentration of 5,188, 3% concentration of 12.242 and 5% concentration of 17,247. The category of SPF values in a row that is medium, maximum and ultra

Flavonoids are natural phenolic compounds found in almost all plants that have potential as antioxidants. Based on previous research, stating that black soybeans contain compounds of flavonoids. This analysis aims to establish the levels of total flavonoids on ethanol extract of black soybean hydrolyzed. Determination of total flavonoid levels of ethanol extract of black soybean hydrolyzed has been made with UV-Vis spectrophotometry method. Black soybean hydrolyzed using HCl and extracted using ethanol 70%. The extracts obtained were analyzed qualitative by the method of coloring reaction and quantitative analysis using UV-Vis spectrophotometer, where quercetin is used as a reference standard. Measurements were performed at maximum wavelength of 444.45 nm. The research showed the average level of total flavonoids is 11.83 mgQE / g extract or 1,163 %. In conclusion extract of black soybean hydrolyzed rich of total flavonoids, and it is related to the activity as antioxidant.

Antioxidants are chemical compounds that can donate one or more electrons to free radical, so that free radicals that can be suppressed.This study aimed to obtain and provide scientific evidence to the public regarding the antioxidant activity in propolis liquid product circulating in Makassar. This study using FRAP (Ferric Reducing Antioxidant Power) and 96% ethanol. Free radical activity absorbance was measured with a UV-VIS spectrophotometer at a wavelength of 710 nm and the total value of antioxidant activity was calculated based on data absorbance. The calculations show that the sample A has antioxidant activity of 0.0971 mgAAE / mg sample, the sample B has antioxidant activity of 0.0669 mgAAE / mg sample, the sample C has antioxidant activity of 0.0493 mgAAE / mg samples.