No abstract

No abstract

No abstract

The dominant trend in molecular genetic methods research of biological samples is the automation of all stages, the most valuable of which is preparation of the sample. The use of microfluidic technologies provides not only full control of all operations at this stage, but also reduces the impact of the environment on the sample preparation process. Magnetic beads technology is one of the methods for extracting RNA and DNA with subsequent analysis. The paper presents magnetic beads-based nucleic acids extraction in the microfluidic chip via automatic mode with further registration by real-time polymerase chain reaction. We have shown that efficiency of DNA extraction in microchip is compared to that in case of conventional manual extraction and allows further improvement by means of automation.

To analyze the results of amplification in the emulsion, special software package was developed. It was used to process images of emulsions, in which digital loop mediated isothermal amplification (LAMP) was carried out. Program units made it possible to combine a set of images of a reaction chamber into a single image file, count the number of droplets in chamber, and obtain histograms of the size distribution and fluorescence intensity of the droplets. The data obtained are necessary at the stage of equipment development for the selection of design parameters and for improving of the experiment methodology.