Essential hypertension is a common disease with fatal clinical complications. Epidemiological and family studies have confirmed the role of genetic predisposition in its development. Hypertensive patients have been shown to have an altered profile of pro- and anti-inflammatory cytokines. The aim of our investigation was to reveal the association of interleukin-6, interleukin-12, and interleukin-10 gene polymorphisms with essential hypertension and its clinical complications in a Tatar ethnic group from Bashkortostan, Russia. The study involved 362 hypertensive patients and 244 healthy subjects from this Tatar ethnic group (Bashkortostan, Russia). DNA was isolated from whole venous blood using phenol-chloroform extraction by the standard method. IL6 -572 G/C, IL12B 1159 C/A, and IL10 -627 C/A genotypes were typed using polymerase chain reaction followed by restriction enzyme digestion. We found that the IL10 -627 *C/*C genotype was associated with decreased risk of hypertension (OR = 0.64, P = 0.035). IL6 genotypes and allele distribution did not differ significantly between subjects with and without hypertension, but the IL6 -572 *G/*G genotype frequency was found to be significantly higher among those patients who had stroke, compared with normotensive control subjects (P = 0.036). Carriers of the IL12B 1159 *A/*A genotype had a lower risk of stroke (OR = 0.38, P = 0.028). Our study has shown the association between IL10 -627 C/A polymorphism and essential hypertension in the Tatar ethnic group from Bashkortostan, Russia. The IL10 -627*C/*C genotype was found to be protective against hypertension. We also demonstrated that hypertensive patients with the IL12B *A/*A and IL6 *G/*G genotypes had increased risk of stroke. Our results suggest a role for cytokines in cardiovascular disease development in the Tatar ethnic group, but further investigation is needed.

No abstract

Башкирский государственный медицинский университет. 450000, Уфа, ул. Ленина, 3Цель. Установить роль иммуновоспалительных реакций и дисфункции эндотелия в ремоделировании миокарда и прогрессировании ишемической болезни сердца (ИБС). Материал и методы. Обследованы 413 больных ИБС, из них 251 -со стабильной и 162 -с нестабильной стенокардией. В контрольную группу включены 55 здо-ровых лиц. Ремоделирование миокарда изучали методом эхокардиографии. Для оценки иммуновоспалительных реакций исследовали показатели первичных и вто-ричных продуктов перекисного окисления липидов (ПОЛ) и антиоксидантных ферментов (АОФ), про-и противовоспалительных цитокинов. О функции эндотелия судили по эндотелийзависимой вазодилатации (ЭЗВД), уровням эндотелина-1 (ЭТ-1) и молекул адгезии. Результаты. При корреляционном анализе выявлены взаимосвязи показателей ПОЛ и АОФ с параметрами провоспалительных цитокинов -интерлейкина-1β, ин-терлейкина-6, фактора некроза опухоли альфа (ФНО-α) (r=-0,39; -0,54; -0,42, соответственно; р<0,05) и величинами ЭЗВД, ЭТ-1 и Е-селектина (r=-0,44; 0,52; 0,49, соответственно; р<0,05). Установлены взаимосвязи продуктов ПОЛ и глутатионпероксидазы с гемодинамическими показателями, определены зависимости между уров-нем ФНО-α и параметрами ремоделирования миокарда. Значимые корреляции выявлены между показателями функции эндотелия и миокарда. Заключение. На основе корреляционного анализа установлена роль активации иммуновоспалительных реакций и дисфункции эндотелия в ремоделировании мио-карда и прогрессировании ИБС. Ключевые слова: ишемическая болезнь сердца, иммуновоспалительные реакции, дисфункция эндотелия, ремоделирование миокарда. Рациональная фармакотерапия в кардиологии 2014;10(5):488-494The role of immune inflammatory reactions and endothelial dysfunction in myocardial remodeling and progression of ischemic heart disease N.E. Zakirova, A.N. Zakirova* Bashkir State Medical University. Lenina ul. 3, Ufa, 450000 Bashkortostan, Russia Aim. To study the role of immune inflammatory reactions and endothelial dysfunction in myocardial remodeling and progression of ischemic heart disease (IHD). Material and methods. 413 patients with IHD including 251 patients with stable and 162 patients with unstable angina were enrolled into the study. 55 healthy subjects were included into control group. Myocardial remodeling was studied by echocardiography. Immune inflammatory reactions were studied by detection of primary and secondary products of lipid peroxidation and antioxidative enzymes, pro-and antiinflammatory cytokines. Endothelial function was assessed by endothelium-dependent vasodilatation (EDVD) and levels of endothelin-1 (ET-1) and adhesion molecules. Results. Correlation analysis showed relations of indicators of lipid peroxidation and antioxidative enzymes with proinflammatory cytokines -interleukin-1β, interleukin-6, tumor necrosis factor-alpha (TGF-α) (r=-0.39; -0.54; -0.42, respectively; р<0.05) and EDVD, ET-1 and E-selectin (r=-0.44; 0.52; 0.49, respectively; р<0.05). Interrelations of lipid peroxidation produc...

Objective: to evaluate the severity of immuno-inflammatory responses under stable stenocardia in patients with ischemic heart disease (IHD). Patients and intervention: the study included 83 patients suffering from IHD. Among them 30 cases were diagnosed as functional class (FC)-II stenocardia, 27 cases as FC-III stenocardia and 26 cases as FC-IV stenocardia. The control group included 25 healthy persons. For characterizing the immuno-inflammatory responses we examined the level of C-reactive protein (CRP), pro-inflammatory (IL-1b, IL-6, TNF-α) and anti-inflammatory (IL-4, IL-10) cytokines by the immunoenzymic procedure. Results: FC-II stenocardia showed normal levels of CRP and pro-inflammatory cytokines. FC-III stenocardia was associated with a moderate increase in markers of an inflammation. FC-IV stenocardia was characterized by maximum levels of CRP and pro-inflammatory cytokines. Conclusion. The intensity of immuno-inflammatory responses depends on more or less serious course of stenocardia in patients with IHD.

No abstract