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Loop-mediated isothermal amplification (LAMP) is widely used as a fast and sensitive diagnostic method and is most promising for analysis in conditions of limited time and resources, for example, in places of care. The paper discusses the basic principles of LAMP technique, methods for detecting reaction products, as well as some microfluidic devices and analytical systems based on LAMP, including those developed for express diagnostics in the context of the COVID-19 pandemic.

The efficiency of silicon-glass chips for the nucleic acids analysis with a concentration of 106-102 copies/µl using various algorithms of searching threshold cycles is shown. Hypotheses that explain the observed PCR efficiency decrease for microfluidic chips compared to polymer test tubes are discussed.

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