Inconsistencies in the ab sorption cross section of ozone have b een ob served. Hence, for accurate measurement, we have reported the comb ined effects of varying optical path -length and temperature on the ozone gas ab sorption cross section (OACS) at 334.15nm. Adopting optical ab sorption spectroscopy, results of the (OACS) have b een simulated using spectralcalc simulator with HITRAN 12 has the latest line list. OACS increased b y 52.27% as the temperature increased from 100K to 350K while it was slightly affected b y a 0.007% decrease varying the path-length from 0.75cm -130cm.