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Supported bilayer lipid membranes (s-BLMs) were formed by the selfassembly method on freshly fractured metallic wires. The influence of a host of compounds in membrane forming solutions on s-BLMs and the effect of pH on the function of the s-BLM were studied. Additionally, the redox reactions of a s-BLM containing vinylferrocene as an electron mediator have been investigated by cyclic voltammetry. The results have shown that ferrocene can be very easily immobilized in s-BLMs. This suggests that s-BLM systems offer a novel approach to the electrode modification by simple way of immobilization of compounds within BLM.