The aim of the study was to investigate and to evaluate the readiness and job satisfaction rates of military medical specialists and civilian personnel of military medical organizations of the Russian Ministry of Defense of central and district subordination (MMO-MOD group), as well as the readiness and job satisfaction indicators of medical specialists from medical organizations of the Russian Ministry of Health and the Federal Medical and Biological Agency (MOH-FMBA group) within the non-staff units of the Disaster Medicine Service for medical and sanitary consequences of emergencies elimination activities. Materials and research methods. Materials of the study: sociological survey questionnaires of the non-staff medical specialists divided into 2 groups (MOH-FMBA group, n = 255; MMO-MOD group, n = 227). The following was assessed: general characteristics of a medical specialist, his/her professional activity at the main place of work and in the non-staff formations when working in high readiness and emergency modes; readiness of non-staff medical specialists to eliminate medical and sanitary consequences of emergencies. Research methods: analytical, logical, statistical (descriptive statistics, correlation and factor analysis). Results of the study and their analysis. Satisfaction with the work of all military medical specialists and civilian personnel of the Russian Ministry of Defense of central and district subordination, as well as medical specialists from medical organizations of the Ministry of Health and the Federal Medical and Biological Agency of Russia were noted. It is marked that among the factors influencing professional activity of medical specialists of different departments in non-staff formations the most important should be considered special training and provision of non-staff formations with medical and other material and technical equipment, readiness of non-staff formations when working in the mode of daily activity to perform tasks on purpose.