Hildegardia species are an important resource for fiber industry. This investigation was conducted to develop a plant regeneration protocol for Hildegardia populifolia (Roxb.) Schott & Endl. via indirect organogenesis Callus was obtained from leaf, internode and petiole explants, among these explants internode explant gave best result on MS medium supplemented with different concentrations of 2,4-Dichlorophenoxy acetic acid (2,4-D). The highest percentage (100%) of regeneration was obtained with benzyladenine (BA) (2.0 mg/l) + indole-3-acetic acid (IAA) (0.1 mg/l) + glutamine (25 mg/l) + thidiazuron (TDZ) (0.5 mg/l) from internode explants. Shootlets were highly rooted on MS medium supplemented with 3.0 mg/l indole-3-butyric acid (IBA). In vitro rooted seedlings were successfully acclimatized. This in vitro regeneration system will facilitate further development of reliable procedures for this genus. ª 2014 Production and hosting by Elsevier B.V. on behalf
The importance of a clean environment is recognized as a “pillar” of sustainable development. There
is, however, a serious public health threat associated with heavy metal releases into the environment.
Aquatic environments in Tamil Nadu state of India lack heavy metals monitoring data. From year
2008 to 2022, research articles were assessed that focused at heavy metal concentrations in freshwater
and marine ecosystems. It has been shown that elevated levels of heavy metals in sediments of aquatic
ecosystems contribute to an increase in their abundance, which may further enter the food chain through
bioaccumulation. In addition, intensifying human exploitation of the South East Coastal region (SEC)
in Tamil Nadu state of India through industries, tourism, aquaculture and recreation further complicates
the situation by providing ever-changing sources of contamination. As a result of analyzing all heavy
metals, the levels of Cd in biota collected from ocean environments were higher. Rivers and lakes have
shown increased levels of heavy metals such as Zn, Cu and Pb as a result of localized anthropogenic
activities. In accordance with sediment guidelines and human health risk assessments, most of the
heavy metals concentrations in sediments and aquatic biota exceeded the permissible limits. It is
highly recommended that more monitoring studies are conducted to monitor the levels of heavy metals
in organisms and in urban rivers, lakes and marine environments in the future, with extreme importance
both for environmental health and economics. By identifying the sources of pollutants and implementing
strict policies to abate pollution, local governments can implement better control measures to decrease
pollution levels.
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