Dioscorea alata, (local name ‘uwi’), is one of the potential local food sources in Central Java, Indonesia. Tubers are still found in several locations in Central Java. This study aimed to characterize the morphology of D. alata, to analyze the antioxidants, starch, amylose, amylopectin, and its potential as a functional food source. The materials for this research were seven variants of D. alata collected from Boyolali, Semarang and Kudus, Central Java. Morphological characterization was approached base on the tuber characteristics. The antioxidant analysis was done by Diphenylpicrylhydrazyl (DPPH) method whereas amylum and amylose analysis were done by spectrophotometry method. There are variations of tuber characters, especially in its shape, exoderm character, flesh color, and texture. D. alata has high antioxidant activity. The comparison of amylose towards amylum is 7.541 – 26.735%, however, the comparison of amylopectin towards amylum is around 73.265 – 92.469%. Uwi ungu Kudus is the best Dioscorea alata variety for diabetics.