Germination of the ascospores of Byssochlamys nivea required a heat shock at 75° for 5 min, and acetate ion. Aeration and pH 4.0–4.5 further enhanced germination. No other trichloroacetic acid cycle compounds were stimulatory, and a survey of 18 amino acids and a number of miscellaneous compounds did not reveal any other germination factors. Extreme variation throughout the investigation suggested that other undisclosed factors were involved.

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