Background: Hair growth promotion via the use of vasodilators represents an important approach in hair loss management as they have proven to be effective in promoting hair growth. Nebivolol is a potent vasodilator which also exhibits anti-apoptotic, anti-inflammatory, and antioxidant effects, yet its role in hair growth induction is not well known. Objective: The present study aims to investigate hair growth-promoting effect of the topical application of nebivolol cream in two strengths 5 and 10% in mice models. Methods: Active hair growth (Anagen) induction by the topical use of nebivolol cream has been evaluated in mouse model. Fifty male swiss albino mice (8 weeks aged) dorsal skins were shaved and these mice were divided randomly and equally into untreated control group with which other experimental groups compression was done and 4 treated groups (n=10) with nebivolol 5% cream, nebivolol 10% cream, minoxidil 2% topical solution and vehicle (1:1 vaseline and lanoline).