NPS standard curveVariables affecting acid hydrolyses were investigated for their efficacy in producing high quality amino acid hydrolysates from crab waste protein for its potential use in specialized animal feed and other food products. In a preliminary trial, SN HCl was found to be a suitable hydrolyzing agent based on the presence of nihydrin-positive substances. Amino acid yields from a 12 hr hydrolysis time varied from about 28-31%, increased marginally to 29-32% with 24 hr of hydrolysis, but decreased with hydrolysis time in excess of 24 hr. Depending upon hydrolysis time, approximately 42-44% of the total amino acids were essential amino acids: principally leucine, arginine, valine, and threonine. The remaining nonessential amino acid fraction was composed mainly of glutamic acid, aspartic acid and glycine.Amino acid standard solution with a strength of 2.5 uM of each amino acid/ml and a total amino acid content of 5.78 mg/mL was used to calibrate the NPS standard curve. The standard solution was diluted with water to get test samples containing 10, 20, 40, 60, 80 and 100 kg of amino acids/ml volume. One half mL of test sample was mixed with 2.5 mL of ninhydrin reagent, let stand in boiling water for 20 min; on cooling made to 13 mL volume with 50% npropanol, read at 570 nm and data used to plot the NPS standard curve.

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SiuJtil(ry. Metabolic changes were sttudied in emllbryvonic axes and cotyledons isolated fromii after-ripeniing seeds of Pru-nus ccrasus. Dutring the sevenith week of after-ripening, a striking increase in the respirationi rate at 250 of embryonic axes occuirre(l along with a sharp chanige from the dormant to non-dormant state of the see(l. On the basis of C-6/C-1 ratio determinatioiis this chanige may be related to an increased activity of the pentose phosphate cvcle. Ba'4C03, plated on microporous porcelain discs, drie(d anid the radioactivity determined by a conitiotis gas flow Geiger coulnter.

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