The reference values for biochemical parameters in blood serum of the zhabe type Kazakh horse are presented in this article. To obtain reference biochemical parameters, whole blood samples of 50 clinically healthy adult zhabe-type horses of both sexes and raised on the free pasture of the "Kalka" farm in Zhambyl region, Shu district, Baluan Sholak, were examined on an automatic biochemical analyzer A15 (Biosystems, Spain). Biochemical results of zhabe type horses were close to the established normal indicators. The levels of enzymes of the hepatobiliary system (aspartate amintransferase, alkaline phosphatase), triglyceride content in horses of the zhabe type were 3-5 times higher than normal. Lower concentrations of albumin and zinc were found (22.22±3.67 g/l and 4.86±1.49 mmol/l, respectively), as well as creatinine concentrations within the lower normal values (77.84±14.19 mmol/L). Therefore, reference intervals of biochemical blood parameters (albumins, total protein, urea, uric acid, cholesterol, creatinine, bilirubin, triglycerides, glucose, calcium, phosphorus, magnesium, iron, zinc, alkaline phosphatase, alanine amintransferase and aspartate amintransferase) were determined for the zhabe type horse in the conditions of the Southern region of the Republic of Kazakhstan, typical for the normal course of metabolic processes in the body.