No abstract

Th e aim of the research. correlation analysis between the DMFT index, the content of the active form of vitamin D – 25(OH)D3 in blood and the level of protective proteins in mixed saliva in young individuals with dental caries. Material and methods. The content of vitamin D metabolite in serum of young individuals with dental caries was evaluated using the chemiluminescent immunoassay method. The concentrations of the antimicrobial peptide – cathelicidin LL-37, alpha-defensin 1-3, secretory immunoglobulin A, lipopolysaccharide binding protein (LBP) in oral fluid were determined using the ELISA method. During dental examination, the analysis of dental caries intensity was conducted through calculating the DMFT index. Statistical processing of the study results was carried out with the “IBM SPSS Statistics Version 25.0” (International Business Machines Corporation, USA) soft ware package. Results. A noticeable inverse relationship between DMFT values, the level of 25(OH)D3 in serum and the values of LBP and sIgA in saliva has been revealed. The content of cathelicidin LL-37, alpha-defensin 1-3, secretory immunoglobulin A and LBP in the oral fl uid of young individuals with dental caries decreases against the background of a low level of serum 25(OH)D3, while noticeable direct correlations between the active form of vitamin D and the value of cathelicidin LL-37, alpha-defensin 1-3 and LBP have been registered. Conclusion. Th e established close relations between the carious process intensity, the volume of 25(OH)D3 in the organism and the immune parameters of saliva confi rm the pathogenetic signifi cance of shift s in the system of oral cavity protection factors.

The content ofММР-9 and ММР-2 in oral fluid of 105 individuals between the ages of 19 and 23 has been researched.Of these, 42 people are individuals with dental caries and normal level of the active form of vitamin Din serum (25(OH)D >30ng/mL) and 42 people - with 25(OH)D <30 ng/mL level.The control group was composed of 21 individuals with low DMFt index (1,5) and a normal level of 25(OH)D in blood. It has been established that the level of ММР-9 in mixed salivaincreases against the background of dental caries,while the content of ММР-9 and ММР-2 increasessignificantlyamidthe lack and deficiency of25(OH)Din the body. Inverse correlations between the 25(OH)D level in serum and the value ofmatrix metalloproteinasesin saliva have been revealed: noticeable - with the amount of MMP-9 and moderate- with the concentration of MMP-2.

No abstract

Oral fluid is a unique biological environment, containing a wide range of substances, coming from local and systemic sources, which makes it possible to use it as an object for assessing pathological changes in the body both at the local and systemic levels. In comparison with the traditional method of blood analysis, the advantage of evaluating the parameters of the oral fluid is the non-invasive of this method of obtaining material. All patients underwent oral fluid sampling using special plastic containers with a swab, which facilitate the selection of material, eliminating the penetration of mucin into a clean test sample, which helps to obtain more accurate analysis results.The amount of secretory IgA, lipopolysaccharide-binding protein (LBP), TBA-active products, the level of total antioxidant activity in the oral fluid in individuals with a low level of 25(OH)D before and after taking the native solution of vitamin D “Aqua Trim” were determined. The concentrations of secretory immunoglobulin A, lipopolysaccharide, binding protein and the level of total antioxidant activity are reduced in the oral fluid of people with vitamin D deficiency, but the number of intermediate products of lyoperoxidation increases. The course intake of the native solution of vitamin D (International Nonproprietary Name - Colecalciferol) normalizes the functioning of the immunity of the oral cavity and restores the balance of the “lipid peroxidation-antioxidants” system.