The method development and validation of Metformin based on UltraViolet spectrophotometry. This compound belongs to the class of organic compounds known as biguanides. These are organic compound containing two N-linked guanidines. Metformin is an oral anti diabetic drug in preventing complications of type 2 diabetes and it is a good first line therapy for a over obese with type 2 diabetes, it is currently available in more than 60countries worldwide. As a result of the importance of this oral hypoglycaemic agent in the treatment of non insulin dependent diabetes mellitus. The reported methods include the chromatographic methods such as HPLC. HPLC has been reported for the determination of Metformin chromatographic methods, these are complex, tedious, time consuming and are not suitable for routine analysis. However, Ultraviolet spectrophotometric methods which are simple and fast for ideal and routine analysis. We observed the maximum absorbance at 235&274nm for Metformin solution ELICO SL-159 make Ultra-Violet visible spectrophotometry spectrum model UV-2450, has been recorded when scanned in the ultraviolet region, using mobile aceto nitrile: phosphate buffer (30:70) with a flow rate of 1.0ml/minute and wave length at 225 nm. Thus, it was selected as the analytical wavelength. The development method was found to be simple, sensitive, accurate, precise and specific as evidenced by non-interference from the excipients presented in the marketed injection.
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