a b s t r a c tIn this study, novel (E)-3-(5-substituted-1H-indol-3-yl)-1- (5,5,8,8-tetramethyl-5,6,7,8-tetrahydronaphthalen-2-yl)prop-2-en-1-one (5(aee)) derivatives were synthesized and their anticancer effects were determined in vitro. Novel indole retinoid compounds except 5e have anti-proliferative capacity in liver, breast and colon cancer cell lines. This anti-proliferative effect was further analyzed in breast cancer cell line panel by using the most potent compound 5a. It was determined that 5a can inhibit proliferation at very low IC 50 concentrations in all of the breast cancer cell lines. Here, we present some evidence on apoptotic termination of cancer cell proliferation which may be primarily driven by the inhibition of RXRa and, to a lesser extent, RXRg.
Background:
Poly (ADP-ribosyl) polymerase-1 (PARP-1) inhibitors are compounds that are used to treat cancers, which are
defective in DNA-repair and DNA Damage-Response (DDR) pathways.
Objective:
In this study, a series of potential PARP-1 inhibitor substituted (piperazine-1-carbonyl)phenyl)-1H- benzo[d]imidazole4-carboxamide compounds were synthesised and tested for their PARP-1 inhibitory and anti-cancer activities.
Methods: Compounds were tested by cell free colorimetric PARP-1 activity and MTT assay in MDA-MB-231, MDA-MB-436,
MDA-MB-468 breast cancer and L929 fibroblast cell lines.
Results:
Our results showed that compound 6a inhibited viability in MDA-MB-231 and MDA-MB-468 cells whereas 8a inhibited
viability in MDA-MB-468 cells. Compound 6b significantly inhibited cell viability in tested cancer cells. However, 6b exhibited
toxicity in L929 cells whereas 6a and 8a were found to be non-toxic for L929 cells. Compounds 6a, 6b and 8a exhibited significant
inhibition of PARP-1 activity.
Conclusion:
These three compounds exhibited PARP-1 inhibitory activities and anticancer effects on breast cancer cells and
further research will enlighten the underlying mechanisms of their effects.
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