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Medicinal plants were shown to be a renewable resource that includes substances with nutritional and pharmaceutical characteristics that enable humans to prevent and treat ailments (El Sohaimy, et al., 2015). Plants are important sources of natural antioxidants that can be used to generate novel medications, and medicinal plants have been designated as a repository for numerous bioactive chemical types with various therapeutic qualities. The therapeutic potential of plants was discovered over a long period of time. The great majority of medicinal plant therapeutic benefits include antioxidants, analgesics, anti-inflammatory, antiviral, anti-tumor and antimalarial characteristics (El Sohaimy, et al., 2015). Bidens pilosa was from the Asteraceae family, also known as compositae and it is the biggest angiospermic family of dicotyledonous plants, owing to the huge number of individuals (1,620 genus and 23,600 species) which make up this global family in the plant kingdom (Funk, et al., 2005). Attard and Cuschieri, (2009) revealed that Terpenoids (particularly sesquiterpene lactones), flavonoids, alkaloids, polyacetylenes, and numerous phenolic compounds are among the phytonutrients identified in this family. the phytochemical analysis demonstrated that alkaloids, saponins, flavonoids, cardiovascular glycosides and tannins are present in the two ARTICLE INFO ABSTRACT Article History