We aimed to determine the profile of IgE reactivity to three major cat allergens, Fel d 1, Fel d 2 and Fel d 4, in cat-allergic patients in the Moscow region in Russia. sIgE levels to recombinant proteins expressed in Escherichia coli (Fel d 1 and Fel d 4) and to Fel d 2 protein purified from cat serum were measured using a microarray method developed in our laboratory. Sera from 174 anonymous subjects with a positive reaction (≥0.35 IU/mL) to cat dander extract (e1, ImmunoCAP) and 56 negative controls were used for IgE testing. Fel d 1 was recognized by 92.5%, Fel d 2 by 29.9% and Fel d 4 by 39.1% of the tested patient sera. The sensitivity to these three proteins was approximately 98% compared to cat dander extract (correlation coefficient to ImmunoCAP is 0.94 with PPV = 0.99 and NPV = 0.95). These predictive values appeared to be even more statistically significant than the divergence between the ISAC IgE test and the extract-based singleplex ImmunoCAP. The combination of the three investigated proteins (Fel d 1, Fel d 2 and Fel d 4) is suitable for in vitro molecular (serological) diagnosis of cat allergy in this region as a complement to cat dander extract. Moreover, with this method, we found distinction between Fel d 2 and other Feline sIgEs formation.

weren't found in reference cohort (50 samples) under assay conditions.From 163 Guinean samples in absence of IgG IgM to arboviruses antigens were found in 16 sera (9.8%), indicating about acute infection stage. From that 16 cases in 4 cases anti-ZIKV IgM were detected, in 2 cases -anti-DV, in 2 cases -to unspecified flavivirus, in 1 case -anti-CHIKV, 4 cases -anti-CCFV, in 2 cases -anti-RVFV. Conclusion:We showed that developed microarray is highly sensitive and specific diagnostic mean. When IgM response to arboviruses antigens is investigated the usage of the technique could be useful for rapid diagnostics as it could help to combine several ELISAs investigations in one test. So the developed microarray could be considered as time-and resource-saving perspective screening test.

This paper describes the protein array as a novel serological test for the diagnosis of Borrelia miyamotoi disease (BMD), by reporting the methodology, the development of a diagnostic algorithm, and its extensive validation. With rising numbers of ticks and tick bites, tick-borne diseases, such as BMD, urgently deserve further societal and medical attention. B. miyamotoi is prevalent in Ixodes ticks across the northern hemisphere.

classified as ZIKV seropositive and the remaining 12 (16.7%) as flavivirus seropositive. Eleven of 14 provinces contributed ZIKV positive specimens, ranging from 4.5% of specimens from North Sumatra, Banten, and East Kalimantan to more than 18% of specimens from Central Java. Overall ZIKV seroprevalence in the 1-4 year-old cohort was 9.1%.Conclusion: We have conducted ZIKV serological screening on samples collected from children in representative areas in Indonesia and revealed the seroprevalence of ZIKV in most regions. The data provide evidence of widespread recent transmission and endemicity of ZIKV in the country. This knowledge provides clues for understanding future patterns of Zika virus transmission in nonendemic areas.