The research introduces a system for pulsed volume discharges ignition at atmospheric pressure within gaps reaching 125 mm. The corona discharge is used for the volume discharge initiation. A damping oscillations pulse generator is used as a high-voltage power supply. The pulse repetition rate reaches 1 kHz, while the rate of damping high-frequency harmonic oscillations can reach megahertz units. The volume discharge electric and spectral characteristics were analyzed. The study revealed that O₂⁺ emission spectrum dominates in the UV region. The potential of using pulsed volume discharge for cleaning biological surfaces was demonstrated in the research. The survival rate for E. coli under the influence of 15 seconds long pulsed volume discharge has decreased by 30 times.