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Three approaches improving the sensitivity of previously established immunoassays for broad detection of sulfonamides (SAs) in milk are described.

The immunological properties of monoclonal autoantibodies to thyroid thyroglobulin in the construction of carrier-bioligand conjugates have been studied. Silver and gold nanoparticles 40-50 nm in size of and polymer microspheres (706 nm) were obtained. Autoantibodies were immobilized on the surface of the synthesized nanoparticles and the effect of nanocarriers on the immunological properties of immobilized monoclonal autoantibodies to thyroglobulin was studied by ELISA. It was found that the use of silver and gold nanoparticles allows you to adjust the sensitivity and specificity of the enzyme-linked immunosorbent assay in order to increase or decrease the detection limit of antigens or antibodies for early laboratory diagnostics of diseases and the creation of new-generation diagnostic test systems. nanoparticles, human thyroid gland thyroglobulin, nanocarriers, silver, gold, polystyrene, immunodiagnostics

Волгоградский государственный медицинский университет, кафедра анатомии человека, кафедра гистологии, цитологии, эмбриологии Описан способ применения азида натрия в различных концентрациях для консервации и фиксации биологических объектов. Околощитовидные железы в концентрации раствора азида натрия 0,5%-го сохраняют свою консистенцию, форму, цвет и структуру на протяжении двух лет.

New opportunities are opening up in the study of the relationship between the antigenic structure of thyroid autoantigens and autoantibodies in the pathogenesis of such autoimmune diseases as Graves’ disease (GD) and autoimmune thyroiditis (AIT) using synthesized recombinant proteins of the thyroid-stimulating hormone receptor (TSHR) and human thyroid peroxidase (TPO). In the present work, the results of cloning of the fragments of the TPO extracellular domain and fragments of the RNA sequence of the α-subunit of TSHR, which do not contain nucleotide substitutions, are demonstrated. Recombinant vectors for the expression of proteins of the α-subunit of thyroid-stimulating hormone receptor and fragments of the TPO extracellular domain have been obtained.