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A simple synthesis method of silver nanoparticles and its application as an active surface-enhanced Raman spectroscopy (SERS) colloid are presented in this work. The photoreduction of AgNO 3 in presence of sodium citrate (NaCit) was carried out by irradiation with different light sources (UV, white, blue, cyan, green, and orange) at room temperature. The evaluation of silver nanoparticles obtained as a function of irradiation time (1-24 h) and light source was followed by UV-visible absorption spectroscopy. This light-modification process results in a colloid with distinctive optical properties that can be related to the size and shape of the particles. The Ag colloids, as prepared, were employed as active colloids in SERS. Pyridine and caffeine were used as test molecules.

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