Four samples of Rutilus rutilus collected in various water bodies of the Azov Sea Basin have been investigated. Individuals were examined during their spawning migration in the spring season of 2021. The differences in some physiological and biochemical parameters between the studied samples were considered. It has been found that the content of lipids in the sexual products of males was higher than in the gonads of females, and the protein was lower. Biochemical analysis of fish blood serum showed differences in the level of albumin between females and males and in the amount of triglycerides in the individuals from different fishing sites. In the liver of the fish from the Vostochno-Akhtarskoe (East Akhtarsk) Hatchery, a low activity of all investigated enzymes of the antioxidant system has been recorded.