This study was initiated to assess the efficacy of some biological materials separately or mixed to control Rhizoctonia root rot disease caused by the fungus Rhizoctonia solani. In vitro efficacy assessment showed; glutathione could inhabit fungal growth up to 100% at concentration 3000 mg/L. Whereas, the bacterium Azospirillum brasilense scored 78.63% inhibitory at 10-5 concentration. The fungal bio-agent Trichoderma viride scored 1.33 highest antagonistic activity 5 days of inoculation on PDA medium. Under greenhouse conditions, (Tr + Az + G+ R. solani) and (Tr + G+ R. solani) combination treatments could decrease R.solani infectivity and disease severity up to 0.00% compared to 73.33 and 68.33% for control treatment, respectively. Similarly, these two treatments could induce systemic acquired resistance (SAR) when scored the highest polyphenol oxidase (PPO) activity 6 and 12d of pathogenic fungus inoculation compared to healthy control. They scored 82.14 and 67.07, 78.12 and 65.33 absorbance increase rate (AIR)/min/g fresh leaf weight, respectively, compared to 41.67, 40.08 for AIR/min/g fresh leaf weight, respectively, for healthy control. Amongst other treatments, (Az + R.solani) scored 11.553% highest protein content compared to 9.433% for healthy control.

This study was conducted to evaluate the efficacy of some chemical and biological substances, individually or in combination in reducing fusarium root rot disease in soybeans caused by the fungus Fusarium solani. The results of laboratory evaluation showed that A. chroococcum bacteria achieved 58.90% inhibition by using dilution 10 -7 . Whereas, the biochar scored 100% inhibition rate at a concentration 4%, while the potassium phosphate (K 2 HPO 4 ) scored a complete and 100% inhibition of the growth of the fungus at of 200 mM concentration. At greenhouse conditions, the mixing treatment A .chroococcum with biochar was performed in the presence of pathogenic fungi, and treatment of K 2 HPO 4 with A.chroococcum and biochar with pathogenic fungi, was the most efficient in reducing the incidence and severity of infection with F. solani which was 0.00 and 0.00% respectively, which did not differ significantly from the treatment of K 2 HPO 4 with A.chroococcum with pathogenic fungi, which was 6.67 and 3.33% respectively, compared to the percentage and severity of infection in the control treatment with, 76.67 and 75.00% respectively, as well as their cause of The fresh and dry weight of the plant was significantly increased among their treatments. The factors also showed an efficiency in inducing systemic resistance by increasing the peroxidase enzyme activity 7 and 14 days of adding the pathogen, compared to the treatment (without pathogenic fungi. The two mixing treatments between K 2 HPO 4 with A .chroococcum and biochar with pathogenic fungi and the addition treatment of mixing A .chroococcum with biochar in the presence of the pathogen were 64.00, 70.00, 63.17 and 68.37 respectively the rate of change in photosynthesis / min / g fresh weight of plant leaves. The rate of change of plant leaves was successively, while the rate of change of the enzyme was achieved by the comparison treatment (without pathogenic fungi), which was 22.27 and 23.33 rate of change by photosynthesis / min / gm fresh weight of plant leaves respectively, followed by the remaining treatments.