No abstract

Eosinophilia associated with solid tumors is an infrequent occurrence. The pathogenesis of tumor-associated eosinophilia is not well understood. Interleukin-5 (IL-5) is a cytokine that has been implicated in the development of eosinophilia in mice and humans. However, there is little data associating IL-5 production with eosinophilia in the presence of tumor. We report, in a patient with locally advanced NSCLC, the presence of excessive eosinophilia and elevated serum IL-5 levels at diagnosis. Immunohistochemical staining of the primary tumor showed large amounts of intracellular IL-5. Both eosinophil count and IL-5 levels normalized after surgical removal of the tumor. Tumor-associated eosinophilia observed in this case is mediated by IL-5. The production of IL-5 is related to the presence of the tumor. Am. J. Hematol. 82:234-237, 2007. V V C 2006 Wiley-Liss, Inc.

Findings support allocating funds to provide CCTT education to PCPs to increase numbers of patients willing to participate in CCTTs.

No abstract

This report describes a cytochemical technique for the demonstration of lysozyme in a variety of cells, including normal human and rabbit neutrophil granules, rabbit lacrimal gland cells and blasts obtained from bone marrow and peripheral blood of patients with myelomonocytic and histiomonocytic leukemia. It utilizes the dis-azo dye Biebrich scarlet which stains lysozyme as well as other basic proteins. The method is made specific for lysozyme by the use of N-acetylglucosamine oligosaccharides obtained from an acid hydrolysate of chitin. These greatly diminish the color reaction obtained when Biebrich scarlet is added to lysozyme, presumably by competing with Biebrich scarlet for the active site of the lysozyme molecule. Cytochemically, this is expressed as a marked diminution in the ability of Biebrich scarlet to stain the cytoplasm of lysozyme-containing cells if these cells have been pretreated with a hydrolysate of chitin. Evidence is presented that this stain is specific for lysozyme rather than a nonspecific reaction between Biebrich scarlet and tissue bases, particularly arginine. SCHOLNIK AND KASS Chemicals, Rochester, N. Y.) and ethyl ether until the ether phase became colorless. The Biebnich scarlet was recrystallized from the N , N-dimethylformamide by pervaporation. Chitin (Miles Laboratories, Kankakee, Ill.) was hydrolyzed in 1 N HCI in a boiling water bath for 1 hr at a concentration of 2.5 mg chitin/ml acid. At the AND KASS TABLE I Tissue Biebnich Scarlet-Methyl Green Stain Sakaguchi Reaction Deamination followed by Biebnich Scarlet-Methyl Green Stain Speece Method Control Chitin Control Chitin Control Chitin Normal peripheral blood +n#{176}