Background: Tudri Surkh (Cheiranthus cheiri) is an important medicinal plant species used in Unani system of medicine. Despite having immense medicinal importance, little information is available on the standardization parameters of the species. For this reason, present work was carried out to establish pharmacognostical standards and generate comprehensive report on the quality control and standardization parameters of Tudri Surkh (Cheiranthus cheiri).Methods: Seeds of the plant were examined using microscopy and macroscopy, physicochemical parameters, extractive values, and fluorescence analysis.Results: The macroscopic, microscopy, and physicochemical parameters of seeds of Tudri Surkh (Cheiranthus cheiri) revealed various diagnostic characteristics in the species.Conclusions: This study provides complete pharmacognostic profile of Tudri Surkh (Cheiranthus cheiri) and hence will be useful for correct identification and authentication of the species for future studies.