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Significance and Impact of the Study: In the present study, we for the first time, report an efficient Agrobacterium tumefaciens-mediated transformation (ATMT) system for a hemibiotrophic fungus, Colletotrichum lindemuthianum. This optimized ATMT protocol is versatile and can be used for the functional genetic studies. Insertional mutagenesis studies produce thousands of mutant lines that need to be evaluated for pathogenicity loss and the traditional methods of pathogenicity assay in bean-anthracnose pathosystem are time consuming and laborious. Hence, we developed a quick and robust screening method that produce consistent results similar to the established bioassay methods. Our optimized ATMT and virulence analysis protocols will prove useful in understanding the bean-anthracnose interface.

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