RESUMENEstudio comparativo de la obtención de extractos crudos y semi-purifícados de lipasa de salvado de arroz.Los extractos crudos de lipasas de salvado de arroz desengrasado fueron obtenidos con tres diferentes soluciones de extracción: 1 -CaCb 0.01 M; 2 -Sacarosa 0.6M, KC110mM, MgCb 1 mM, Ditiotreitol 2mM; 3 -Sacarosa 0.4M, Ditiotreitol 2mM. Las variedades estudiadas pH (6.0, 7.0, 8.0) y tiempo de extracción (1h, 2h, 3h). La temperatura de extracción fue de 4 °C. Los extractos obtenidos presentaron diferentes actividades de lipasa siendo el extracto crudo obtenido con CaCl2 0.01 M el que mostró la mayor actividad específica (12. Imü/mg). La purificación parcial de este extracto crudo con acetona y sulfato amónico resultó en fracciones con actividad específica de 50.7mU/mg y 63.5mU/mg respectivamente. PALABRAS-CLAVE: Enzima -Extracción -Lipasa -Purificación -Salvado de arroz. SUMMARYComparative study of the obtention of crude and semi-purified lipase extracts from rice bran.Crude lipase extracts from defatted rice bran were obtained using three different extraction solutions: 1 -CaCb 0.01 M; 2 -Sacarose 0.6M, KCI lOmM, MgCb ImM, Ditiotreitol 2mM and 3 -Sacarose 0.4M, Ditiotreitol 2mM. Factors (variables) studied were pH (6.0, 7.0, 8.0) and extraction time (1h, 2h, 3h). Extraction temperature was 4 °C. The extracts obtained presented different lipase activities, and the crude extract obtained with CaCb 0.01 M was the one with the highest specific activity (12.1mU/mg). Partial purification of this crude extract with acetone and ammonium sulfate resulted in fractions with specific activities of 50.7mU/mg and 63.5mU/mg, respectively KEY-WORDS: Enzyme -Extraction -Lipase -PurificationRice bran.
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